Hotspot

ndhH

SSC · 126862-128043 · 1182 bp

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Pi 0.502

MarkerSeek score 80.10

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 489
pi: 0.502
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	TCAGGTCCAATACGCTGTTG	GAAAACCCGGGATCGATTGA	50.0	50.0	57.9	58.0	1699 bp	0.5	59.0
ndhH_p2	TCAGGTCCAATACGCTGTTG	AGAAAACCCGGGATCGATTG	50.0	50.0	57.9	58.0	1700 bp	0.5	58.9
ndhH_p3	TCAGGTCCAATACGCTGTTG	CTGCGAAGACAAATCGATGC	50.0	50.0	57.9	57.9	2099 bp	0.5	58.6
ndhH_p4	TCAGGTCCAATACGCTGTTG	CGGGAGCCACAAACTCATTA	50.0	50.0	57.9	57.9	1501 bp	0.5	58.4
ndhH_p5	TCAGGTCCAATACGCTGTTG	AAGAAGAAAACCCGGGATCG	50.0	50.0	57.9	57.9	1704 bp	0.5	58.4