Hotspot

ndhA

SSC · 124418-126860 · 2443 bp

Back to Pentaclethra

Pi 0.455

MarkerSeek score 78.90

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2443
variable_sites: 949
pi: 0.455
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CCTTGCGGCTCGTACTATTT	GTGAGAGTTGAAGCCCCAAA	50.0	50.0	58.1	58.0	2786 bp	0.5	59.3
ndhA_p2	CCTTGCGGCTCGTACTATTT	AAAAAGCCGTCTCCGACTTT	50.0	45.0	58.1	58.0	2831 bp	0.5	59.2
ndhA_p3	CCTTGCGGCTCGTACTATTT	CAGGCTCTGGAAGGAATTCC	50.0	55.0	58.1	58.0	2939 bp	0.5	59.1
ndhA_p4	TACCTTGCGGCTCGTACTAT	GTGAGAGTTGAAGCCCCAAA	50.0	50.0	58.3	58.0	2788 bp	0.5	57.3
ndhA_p5	CCTTGCGGCTCGTACTATTT	TGAGAGTTGAAGCCCCAAAG	50.0	50.0	58.1	57.7	2785 bp	0.5	57.3