Hotspot

ccsA

SSC · 118947-119918 · 972 bp

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Pi 0.477

MarkerSeek score 78.10

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 349
pi: 0.477
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.75
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	GGTATGAGCGGTTTTGTTGC	50.0	50.0	58.0	58.0	1763 bp	0.5	59.8
ccsA_p2	TGAAATCGGTAGACACGCTG	GGTATGAGCGGTTTTGTTGC	50.0	50.0	58.0	58.0	1762 bp	0.5	59.7
ccsA_p3	ACCCCATTTTGGAATTGGCT	GGTATGAGCGGTTTTGTTGC	45.0	50.0	58.0	58.0	1939 bp	0.5	59.5
ccsA_p4	GTGAAATCGGTAGACACGCT	GGTGTTTGTGAGAACCCCTT	50.0	50.0	58.0	57.9	1280 bp	0.5	59.4
ccsA_p5	TGAAATCGGTAGACACGCTG	GGTGTTTGTGAGAACCCCTT	50.0	50.0	58.0	57.9	1279 bp	0.5	59.4