Hotspot

ndhF-rpl32

SSC · 117276-118186 · 911 bp

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Pi 0.427

MarkerSeek score 80.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 911
variable_sites: 321
pi: 0.427
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAAACGCTAGGAAAAGCCCA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.0	58.0	1661 bp	0.5	59.9
ndhF-rpl32_p2	AAAACGCTAGGAAAAGCCCA	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.0	58.0	1662 bp	0.5	59.8
ndhF-rpl32_p3	AAAACGCTAGGAAAAGCCCA	AGCCAATTCCAAAATGGGGT	45.0	45.0	58.0	58.0	1485 bp	0.5	59.6
ndhF-rpl32_p4	TAGGGCGTGTGAACCAAAAA	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	2032 bp	0.5	59.5
ndhF-rpl32_p5	TAGGGCGTGTGAACCAAAAA	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.9	58.0	2033 bp	0.5	59.5