Hotspot

petB-petD

LSC · 80683-80873 · 191 bp

Back to Pentaclethra

Pi 0.0824

MarkerSeek score 89.40

Primer available yes

Label petB-petD

Feature Metadata

label_name: petB-petD
feature_type: igs
region: LSC
length_bp: 191
variable_sites: 15
pi: 0.0824
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 89.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-petD_p1	TAAGCAAGGCATTTCCGGTC	GTTCAAGTCACACACTCCCA	50.0	50.0	58.5	57.7	240-247 bp	1.0	81.0
petB-petD_p2	TAAGCAAGGCATTTCCGGTC	AAGTCATATATCTGCGCGGC	50.0	50.0	58.5	58.3	270-277 bp	1.0	80.4
petB-petD_p3	CAAGGCATTTCCGGTCCTTT	GTTCAAGTCACACACTCCCA	50.0	50.0	58.7	57.7	236-243 bp	1.0	79.5
petB-petD_p4	CAAGGCATTTCCGGTCCTTT	AAGTCATATATCTGCGCGGC	50.0	50.0	58.7	58.3	266-273 bp	1.0	78.9
petB-petD_p5	GTAAGCAAGGCATTTCCGGT	AAGTCATATATCTGCGCGGC	50.0	50.0	58.8	58.3	271-278 bp	1.0	78.1