Hotspot

ndhC-trnV-UAC

LSC · 54544-55145 · 602 bp

Back to Pentaclethra

Pi 0.0776

MarkerSeek score 80.20

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 602
variable_sites: 45
pi: 0.0776
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	AGAGAAGCGGCCATAAACTG	AAAAGATGCACGTTGGGTCT	50.0	45.0	58.0	58.0	1224-1225 bp	1.0	84.2
ndhC-trnV-UAC_p2	CGCACTAAAGAAGGAGCCAT	AAAAGATGCACGTTGGGTCT	50.0	45.0	58.0	58.0	1381-1382 bp	1.0	83.7
ndhC-trnV-UAC_p3	CGTGGTACCAGTCCATAACG	AAAAGATGCACGTTGGGTCT	55.0	45.0	58.1	58.0	1306-1307 bp	1.0	83.5
ndhC-trnV-UAC_p4	TGGTTCAGGCATTTGCTCAT	AAAAGATGCACGTTGGGTCT	45.0	45.0	58.1	58.0	1406-1407 bp	1.0	83.3
ndhC-trnV-UAC_p5	AGAGAAGCGGCCATAAACTG	GCACAAGGGCTATAGCTCAG	50.0	55.0	58.0	58.1	1815-1816 bp	1.0	81.9