Hotspot

rps16

LSC · 147590-148770 · 1181 bp

Back to Penstemon

Pi 0.00999

MarkerSeek score 51.80

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1181
variable_sites: 17
pi: 0.00999
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AAAGCCGAGTACTCTACCGT	TCGTGTTTGGCTCGAATGAA	50.0	45.0	58.2	58.1	2513-2528 bp	1.0	80.1
rps16_p2	AAAGCCGAGTACTCTACCGT	TTCGTGTTTGGCTCGAATGA	50.0	45.0	58.2	58.1	2514-2529 bp	1.0	80.1
rps16_p3	AAGCCGAGTACTCTACCGTT	TCGTGTTTGGCTCGAATGAA	50.0	45.0	58.2	58.1	2512-2527 bp	1.0	80.1
rps16_p4	AAGCCGAGTACTCTACCGTT	TTCGTGTTTGGCTCGAATGA	50.0	45.0	58.2	58.1	2513-2528 bp	1.0	80.1
rps16_p5	AAGCCGAGTACTCTACCGTT	AGCCGGGAGTGACTTATCTT	50.0	50.0	58.2	58.1	2467-2482 bp	1.0	80.1