Hotspot

trnH-GUG-psbA

LSC · 142813-143058 · 246 bp

Back to Penstemon

Pi 0.0612

MarkerSeek score 72.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 246
variable_sites: 20
pi: 0.0612
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	TGGGTAGAGCCGGATCTAAG	TCAGTGCTATGCATGGTTCC	55.0	50.0	58.0	58.0	1088-1095 bp	1.0	85.1
trnH-GUG-psbA_p2	TGGGTAGAGCCGGATCTAAG	GAGCTTAGTTTCCGTCTGGG	55.0	55.0	58.0	58.0	1326-1333 bp	1.0	84.3
trnH-GUG-psbA_p3	TGGGTAGAGCCGGATCTAAG	GAGTGGGAGCTTAGTTTCCG	55.0	55.0	58.0	58.0	1332-1339 bp	1.0	84.3
trnH-GUG-psbA_p4	TGGGTAGAGCCGGATCTAAG	GGAGCTTAGTTTCCGTCTGG	55.0	55.0	58.0	58.0	1327-1334 bp	1.0	84.3
trnH-GUG-psbA_p5	TGGGTAGAGCCGGATCTAAG	GGGAGCTTAGTTTCCGTCTG	55.0	55.0	58.0	58.0	1328-1335 bp	1.0	84.3