Hotspot

ndhG-ndhI

SSC · 107564-107929 · 366 bp

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Pi 0.0323

MarkerSeek score 66.70

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 366
variable_sites: 16
pi: 0.0323
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	AAGACCCGATCCCAGAAAGA	TGACGATTACACAATTCGAACA	50.0	36.4	58.0	56.4	475-481 bp	1.0	55.3
ndhG-ndhI_p2	TCCCAATGAAAAGGCCGAAT	TGACGATTACACAATTCGAACA	45.0	36.4	57.8	56.4	538-544 bp	1.0	53.3
ndhG-ndhI_p3	AAGACCCGATCCCAGAAAGA	TGACGATTACACAATTCGAACAA	50.0	34.8	58.0	57.1	475-481 bp	1.0	52.7
ndhG-ndhI_p4	AAGACCCGATCCCAGAAAGA	TTGACGATTACACAATTCGAACA	50.0	34.8	58.0	57.1	476-482 bp	1.0	52.7
ndhG-ndhI_p5	ACCCGATCCCAGAAAGACTA	TGACGATTACACAATTCGAACA	50.0	36.4	57.5	56.4	472-478 bp	1.0	51.3