Hotspot

ndhF-rpl32

SSC · 101442-101974 · 533 bp

Back to Penstemon

Pi 0.0176

MarkerSeek score 57.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 533
variable_sites: 14
pi: 0.0176
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTAAAGGGATGATCCACGCA	TGATGCAGAAGTACGTTTTT	50.0	35.0	57.4	53.5	604-606 bp	1.0	42.1
ndhF-rpl32_p2	CTAAAGGGATGATCCACGCA	TTGATGCAGAAGTACGTTTT	50.0	35.0	57.4	53.5	605-607 bp	1.0	42.1
ndhF-rpl32_p3	CTAAAGGGATGATCCACGCA	TTTGATGCAGAAGTACGTTT	50.0	35.0	57.4	53.5	606-608 bp	1.0	42.1
ndhF-rpl32_p4	CTAAAGGGATGATCCACGCA	TTTTGATGCAGAAGTACGTT	50.0	35.0	57.4	53.5	607-609 bp	1.0	42.1
ndhF-rpl32_p5	CTAAAGGGATGATCCACGCA	TTTTTGATGCAGAAGTACGT	50.0	35.0	57.4	53.5	608-610 bp	1.0	42.1