Hotspot

rps19-rpl2

IRb · 73756-73818 · 63 bp

Back to Penstemon

Pi 0.0328

MarkerSeek score 76.10

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 63
variable_sites: 3
pi: 0.0328
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	AGATGATTGGCTACAAAAGGGT	GATTGTTCGTCGCCGTAGTA	40.9	50.0	57.7	57.8	130-132 bp	1.0	63.1
rps19-rpl2_p2	AGATGATTGGCTACAAAAGGGT	TTTGATTGTTCGTCGCCGTA	40.9	45.0	57.7	58.2	133-135 bp	1.0	62.7
rps19-rpl2_p3	AGATGATTGGCTACAAAAGGGT	AATTTGATTGTTCGTCGCCG	40.9	45.0	57.7	57.8	135-137 bp	1.0	62.4
rps19-rpl2_p4	AGATGATTGGCTACAAAAGGGT	CACAACCCCTTGGGGTTATC	40.9	55.0	57.7	58.2	199-201 bp	1.0	62.2
rps19-rpl2_p5	AGATGATTGGCTACAAAAGGGT	TTGGGGTTATCCTGCACTTG	40.9	50.0	57.7	57.8	190-192 bp	1.0	61.8