Hotspot

trnG-GCC-trnM-CAU

LSC · 26269-26437 · 169 bp

Back to Penstemon

Pi 0.0454

MarkerSeek score 77.30

Primer available yes

Label trnG-GCC-trnM-CAU

Feature Metadata

label_name: trnG-GCC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 169
variable_sites: 11
pi: 0.0454
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnM-CAU_p1	AGAAGATGCGGGTTCGATTC	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	236-240 bp	1.0	82.9
trnG-GCC-trnM-CAU_p2	AGAAGATGCGGGTTCGATTC	TTACTTCTTCTCTTCGGCGC	50.0	50.0	58.1	58.0	293-297 bp	1.0	82.5
trnG-GCC-trnM-CAU_p3	AGAAGATGCGGGTTCGATTC	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	58.1	58.0	272-276 bp	1.0	82.5
trnG-GCC-trnM-CAU_p4	GAGAAGATGCGGGTTCGATT	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	58.1	58.0	273-277 bp	1.0	82.5
trnG-GCC-trnM-CAU_p5	GAGAAGATGCGGGTTCGATT	TTACTTCTTCTCTTCGGCGC	50.0	50.0	58.1	58.0	294-298 bp	1.0	82.4