Hotspot

atpH-atpI

LSC · 3473-4453 · 981 bp

Back to Penstemon

Pi 0.0144

MarkerSeek score 59.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 981
variable_sites: 21
pi: 0.0144
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACGGAAGCAGCAGAAATCA	TCCCTGTCATGTTCCTTGGA	45.0	50.0	58.1	58.3	1110-1115 bp	1.0	79.1
atpH-atpI_p2	GCCAATCCAGCAGCAATAAC	TCCCTGTCATGTTCCTTGGA	50.0	50.0	57.8	58.3	1127-1132 bp	1.0	78.2
atpH-atpI_p3	TCCAGCAGCAATAACGGAAG	TCCCTGTCATGTTCCTTGGA	50.0	50.0	58.3	58.3	1122-1127 bp	1.0	77.8
atpH-atpI_p4	AACGGAAGCAGCAGAAATCA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.1	58.5	1062-1067 bp	1.0	77.1
atpH-atpI_p5	ATCCAGCAGCAATAACGGAA	TCCCTGTCATGTTCCTTGGA	45.0	50.0	57.6	58.3	1123-1128 bp	1.0	76.6