Hotspot

psbA

LSC · 282-1343 · 1062 bp

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Pi 0.000942

MarkerSeek score 29.80

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 2
pi: 0.000942
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GATCCACTTGGCTACATCCG	CCTGTCTACGAACGGAACTG	55.0	55.0	58.1	58.0	1734-1745 bp	1.0	80.6
psbA_p2	CAATCCACTGCCTTGATCCA	CCTGTCTACGAACGGAACTG	50.0	55.0	57.9	58.0	1748-1759 bp	1.0	80.6
psbA_p3	AATCCACTGCCTTGATCCAC	CCTGTCTACGAACGGAACTG	50.0	55.0	57.9	58.0	1747-1758 bp	1.0	80.6
psbA_p4	ACAATCCACTGCCTTGATCC	CCTGTCTACGAACGGAACTG	50.0	55.0	57.9	58.0	1749-1760 bp	1.0	80.6
psbA_p5	GATCCACTTGGCTACATCCG	ATGCAGCAAGGGTTCATCAA	55.0	45.0	58.1	58.1	1769-1780 bp	1.0	80.2