Hotspot

ndhH

SSC · 122610-123791 · 1182 bp

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Pi 0.00482
MarkerSeek score 59.50
Primer available yes
Label ndhH

Feature Metadata

label_name
ndhH
feature_type
gene
region
SSC
length_bp
1182
variable_sites
12
pi
0.00482
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhH_p1 CCCAATACAGGAGTCAAAATAGGA CGCGTTAATTTAAGTTTGAAGGG 41.7 39.1 58.0 57.0 1408-1416 bp 1.0 41.5
ndhH_p2 TCCCAATACAGGAGTCAAAATAGG CGCGTTAATTTAAGTTTGAAGGG 41.7 39.1 58.0 57.0 1409-1417 bp 1.0 41.5
ndhH_p3 TCCCAATACAGGAGTCAAAA CGCGTTAATTTAAGTTTGAAGGG 40.0 39.1 53.8 57.0 1409-1417 bp 1.0 41.5
ndhH_p4 TTCCCAATACAGGAGTCAAA CGCGTTAATTTAAGTTTGAAGGG 40.0 39.1 53.8 57.0 1410-1418 bp 1.0 41.5
ndhH_p5 TTCCCAATACAGGAGTCAAAA CGCGTTAATTTAAGTTTGAAGGG 38.1 39.1 54.7 57.0 1410-1418 bp 1.0 41.5