Hotspot

ndhF-rpl32

SSC · 112756-113635 · 880 bp

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Pi 0.0198

MarkerSeek score 64.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 880
variable_sites: 34
pi: 0.0198
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGTTGCCGTGGGAAAAAGTA	CGCTGCCCAATATCCTTTCT	45.0	50.0	57.9	58.0	1030-1063 bp	1.0	85.7
ndhF-rpl32_p2	CCGGGTGAACCAAAAACCTA	CGCTGCCCAATATCCTTTCT	50.0	50.0	58.0	58.0	1435-1468 bp	1.0	84.5
ndhF-rpl32_p3	CCGGGTGAACCAAAAACCTA	AACGCTGCCCAATATCCTTT	50.0	45.0	58.0	57.9	1437-1470 bp	1.0	83.6
ndhF-rpl32_p4	CTCCAACGCGATTAGTCACA	CGCTGCCCAATATCCTTTCT	50.0	50.0	58.0	58.0	1487-1520 bp	0.8	76.0
ndhF-rpl32_p5	CTCCAACGCGATTAGTCACA	AACGCTGCCCAATATCCTTT	50.0	45.0	58.0	57.9	1489-1522 bp	0.8	75.1