Hotspot

accD-psaI

LSC · 59313-59980 · 668 bp

Back to Pellionia

Pi 0.0166

MarkerSeek score 66.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 668
variable_sites: 20
pi: 0.0166
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TTGATCCAATCGTACCACGT	AAACCATAGCAATTGCCGGA	45.0	45.0	57.0	58.2	848-874 bp	1.0	74.4
accD-psaI_p2	TTGATCCAATCGTACCACGT	AACCATAGCAATTGCCGGAA	45.0	45.0	57.0	58.2	847-873 bp	1.0	74.4
accD-psaI_p3	TTGATCCAATCGTACCACGT	ACCATAGCAATTGCCGGAAA	45.0	45.0	57.0	58.2	846-872 bp	1.0	74.4
accD-psaI_p4	TTGATCCAATCGTACCACGT	GAAACCATAGCAATTGCCGG	45.0	50.0	57.0	57.8	849-875 bp	1.0	74.0
accD-psaI_p5	TTGATCCAATCGTACCACGT	GCGGGTAAGTTGCTGATAGT	45.0	50.0	57.0	57.7	798-824 bp	1.0	73.4