Hotspot

trnT-UGU-trnL-UAA

LSC · 47187-47798 · 612 bp

Back to Pellionia

Pi 0.0203

MarkerSeek score 56.30

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 612
variable_sites: 23
pi: 0.0203
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GACCACCGCATTACAAATGC	ACCAATTTCGCCATATCCCC	50.0	50.0	58.1	58.0	680-692 bp	1.0	82.2
trnT-UGU-trnL-UAA_p2	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	55.0	50.0	57.9	58.0	662-674 bp	1.0	81.9
trnT-UGU-trnL-UAA_p3	GACCACCGCATTACAAATGC	TAGCGTCTACCAATTTCGCC	50.0	50.0	58.1	58.1	688-700 bp	1.0	81.6
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	55.0	50.0	57.9	58.1	670-682 bp	1.0	81.4
trnT-UGU-trnL-UAA_p5	GACCACCGCATTACAAATGC	CGCTCGGTAGGTTTCCATAC	50.0	55.0	58.1	58.2	735-747 bp	1.0	80.8