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psbZ-trnG-GCC

LSC · 36234-36748 · 515 bp

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Pi 0.0167

MarkerSeek score 53.50

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 515
variable_sites: 16
pi: 0.0167
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTTCTCCTGATGGGTGGTTG	AATCGAACCCGCATCTTCTC	55.0	50.0	57.9	58.1	679-684 bp	1.0	80.7
psbZ-trnG-GCC_p2	CTTCTCCTGATGGGTGGTTG	GAATCGAACCCGCATCTTCT	55.0	50.0	57.9	58.1	680-685 bp	1.0	80.7
psbZ-trnG-GCC_p3	CTTCTCCTGATGGGTGGTTG	CATCTTCTCCGTGGCAAAGA	55.0	50.0	57.9	57.9	668-673 bp	1.0	80.4
psbZ-trnG-GCC_p4	TTTGCTTCTCCTGATGGGTG	AATCGAACCCGCATCTTCTC	50.0	50.0	57.8	58.1	683-688 bp	1.0	80.0
psbZ-trnG-GCC_p5	TTTGCTTCTCCTGATGGGTG	GAATCGAACCCGCATCTTCT	50.0	50.0	57.8	58.1	684-689 bp	1.0	80.0