Hotspot

rpoB-trnC-GCA

LSC · 27007-28048 · 1042 bp

Back to Pellionia

Pi 0.0154

MarkerSeek score 56.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1042
variable_sites: 31
pi: 0.0154
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GTTCTTCTGTTAAGCCCCGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1207-1218 bp	1.0	77.8
rpoB-trnC-GCA_p2	AGTTCTTCTGTTAAGCCCCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1208-1219 bp	1.0	77.7
rpoB-trnC-GCA_p3	GTTCTTCTGTTAAGCCCCGA	TTGTTTATCAGGCGACACCC	50.0	50.0	57.5	58.2	1225-1236 bp	1.0	76.9
rpoB-trnC-GCA_p4	AGTTCTTCTGTTAAGCCCCG	TTGTTTATCAGGCGACACCC	50.0	50.0	57.5	58.2	1226-1237 bp	1.0	76.9
rpoB-trnC-GCA_p5	CTTCTGTTAAGCCCCGATCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.3	58.1	1204-1215 bp	1.0	76.0