Hotspot

trnK-UUU-rps16

LSC · 4242-5022 · 781 bp

Back to Pellionia

Pi 0.0197
MarkerSeek score 60.60
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
781
variable_sites
31
pi
0.0197
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCGCGGTCTTACAAACTC TGAACTTTCGCCTTAATCACT 50.0 38.1 57.9 55.5 915-951 bp 1.0 55.0
trnK-UUU-rps16_p2 GTCGCGGTCTTACAAACTCT TGAACTTTCGCCTTAATCACT 50.0 38.1 57.9 55.5 914-950 bp 1.0 55.0
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT TGAACTTTCGCCTTAATCACT 50.0 38.1 58.2 55.5 832-868 bp 1.0 54.4
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TGAACTTTCGCCTTAATCACT 50.0 38.1 58.2 55.5 831-867 bp 1.0 54.4
trnK-UUU-rps16_p5 CAGTCGCGGTCTTACAAACT TGAACTTTCGCCTTAATCACT 50.0 38.1 58.2 55.5 916-952 bp 1.0 53.7