Hotspot

trnH-GUG-psbA

LSC · 77-281 · 205 bp

Back to Pellionia

Pi 0.0546
MarkerSeek score 73.40
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
205
variable_sites
22
pi
0.0546
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CTGACGGTATGCCTCTAGGA 55.0 55.0 58.1 58.0 783-789 bp 1.0 81.3
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG TCTGACGGTATGCCTCTAGG 55.0 55.0 58.1 58.0 784-790 bp 1.0 81.3
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA CTGACGGTATGCCTCTAGGA 50.0 55.0 57.9 58.0 797-803 bp 1.0 81.2
trnH-GUG-psbA_p4 CAATCCACTGCCTTGATCCA TCTGACGGTATGCCTCTAGG 50.0 55.0 57.9 58.0 798-804 bp 1.0 81.2
trnH-GUG-psbA_p5 AATCCACTGCCTTGATCCAC CTGACGGTATGCCTCTAGGA 50.0 55.0 57.9 58.0 796-802 bp 1.0 81.2