Hotspot

ycf66

Genome · 4584-5493 · 910 bp

Pi 0

MarkerSeek score 58.90

Primer available yes

Label ycf66

Feature Metadata

label_name: ycf66
feature_type: gene
region: Genome
length_bp: 910
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf66_p1	TTTTTCAGCAGATGCAACGG	AAACAAACCCCAGTCGATCC	45.0	50.0	57.6	58.1	1086-1087 bp	1.0	76.0
ycf66_p2	TTTTTCAGCAGATGCAACGG	GGAAATTGAATCCCGTGACG	45.0	50.0	57.6	57.2	1008-1009 bp	1.0	70.1
ycf66_p3	TTTTTCAGCAGATGCAACGG	TGCCTACCAGTATTTTTGCGA	45.0	42.9	57.6	57.9	1044-1045 bp	1.0	68.2
ycf66_p4	TTTTTCAGCAGATGCAACGG	TAAACAAACCCCAGTCGATCC	45.0	47.6	57.6	57.9	1087-1088 bp	1.0	68.2
ycf66_p5	TTTTCAGCAGATGCAACGGG	AAACAAACCCCAGTCGATCC	50.0	50.0	59.4	58.1	1085-1086 bp	1.0	68.1