Hotspot

rps12

Genome · 371-1101 · 731 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label rps12

Feature Metadata

label_name
rps12
feature_type
gene
region
Genome
length_bp
731
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12_p1 TACGGTTCCGTAGAGTGACA TCTGCGATACTTTTACGTGACA 50.0 40.9 57.8 57.8 834 bp 1.0 61.2
rps12_p2 TACGGTTCCGTAGAGTGACA TGGATCAGGCTTTCCTACTT 50.0 45.0 57.8 55.8 859 bp 1.0 61.1
rps12_p3 TACGGTTCCGTAGAGTGACA TTGGATCAGGCTTTCCTACT 50.0 45.0 57.8 55.8 860 bp 1.0 61.1
rps12_p4 ACGAGGAGCCGTATGAAATG TCTGCGATACTTTTACGTGACA 50.0 40.9 57.8 57.8 865 bp 1.0 60.9
rps12_p5 ATTGAACGAGGAGCCGTATG TCTGCGATACTTTTACGTGACA 50.0 40.9 57.8 57.8 870 bp 1.0 60.9