Pi
0.000313
MarkerSeek score
55.50
Primer available
yes
Label
rpoB
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| rpoB_p1 | CAGCATCGAATGAGAGGGAG | TGATTGGGATAGCGTCTTCG | 55.0 | 50.0 | 57.8 | 57.5 | 234 bp | 1.0 | 75.1 |
| rpoB_p2 | CTCCAGCATCGAATGAGAGG | TGATTGGGATAGCGTCTTCG | 55.0 | 50.0 | 57.8 | 57.5 | 237 bp | 1.0 | 75.1 |
| rpoB_p3 | ACGCACGGATTTTATCACGT | TGATTGGGATAGCGTCTTCG | 45.0 | 50.0 | 58.3 | 57.5 | 212 bp | 1.0 | 74.0 |
| rpoB_p4 | GGAGGTACGCACGGATTTTA | TGATTGGGATAGCGTCTTCG | 50.0 | 50.0 | 57.7 | 57.5 | 218 bp | 1.0 | 73.9 |
| rpoB_p5 | TCGAATGAGAGGGAGGTACG | TGATTGGGATAGCGTCTTCG | 55.0 | 50.0 | 58.3 | 57.5 | 229 bp | 1.0 | 73.7 |