Hotspot

atpI

Genome · 17330-18076 · 747 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label atpI

Feature Metadata

label_name
atpI
feature_type
gene
region
Genome
length_bp
747
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI_p1 ACATTTCCGGAGAGAGCAAC GCCATCGTCGACCCAATAAT 50.0 50.0 57.9 58.1 797 bp 1.0 78.2
atpI_p2 ACATTTCCGGAGAGAGCAAC CAATGAAAGCCATCGTCGAC 50.0 50.0 57.9 57.6 805 bp 1.0 75.7
atpI_p3 ACATTTCCGGAGAGAGCAAC TGTGAATCGATTCGACGACG 50.0 50.0 57.9 58.5 832 bp 1.0 75.4
atpI_p4 AAGGAATGTTGGCGAGGAAT GCCATCGTCGACCCAATAAT 45.0 50.0 57.5 58.1 838 bp 1.0 74.9
atpI_p5 AGGAATGTTGGCGAGGAATT GCCATCGTCGACCCAATAAT 45.0 50.0 57.5 58.1 837 bp 1.0 74.9