Hotspot

rps3-rpl22

Genome · 78148-78221 · 74 bp

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Pi 0
MarkerSeek score 56.50
Primer available yes
Label rps3-rpl22

Feature Metadata

label_name
rps3-rpl22
feature_type
igs
region
Genome
length_bp
74
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3-rpl22_p1 TTGCGAACCAGTATGAACGA TCAACCTAGAGCTCAGGGAC 45.0 55.0 57.6 58.1 226-232 bp 1.0 77.4
rps3-rpl22_p2 TGCGAACCAGTATGAACGAT TCAACCTAGAGCTCAGGGAC 45.0 55.0 57.3 58.1 225-231 bp 1.0 75.6
rps3-rpl22_p3 TTGCGAACCAGTATGAACGA AGGGACGCGGTTATCCTATA 45.0 50.0 57.6 57.4 212-218 bp 1.0 74.0
rps3-rpl22_p4 TTGCGAACCAGTATGAACGA TTCAACCTAGAGCTCAGGGA 45.0 50.0 57.6 57.4 227-233 bp 1.0 73.6
rps3-rpl22_p5 TTGCGAACCAGTATGAACGA GGGACGCGGTTATCCTATAC 45.0 55.0 57.6 57.4 211-217 bp 1.0 73.6