Hotspot

clpP-psbB

Genome · 68154-68513 · 360 bp

Back to Pellia

Pi 0
MarkerSeek score 25.20
Primer available yes
Label clpP-psbB

Feature Metadata

label_name
clpP-psbB
feature_type
igs
region
Genome
length_bp
360
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
25.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP-psbB_p1 AAAGGAACCTTCGGAACACC GCACAGCAATTAAACGACCC 50.0 50.0 58.0 58.0 456 bp 1.0 79.6
clpP-psbB_p2 GGAACCTTCGGAACACCAAT GCACAGCAATTAAACGACCC 50.0 50.0 58.1 58.0 453 bp 1.0 79.1
clpP-psbB_p3 AAAGGAACCTTCGGAACACC GACGCGATACCAAGGTAGAC 50.0 55.0 58.0 57.9 413 bp 1.0 78.9
clpP-psbB_p4 GACGAAAAGGAACCTTCGGA GCACAGCAATTAAACGACCC 50.0 50.0 57.8 58.0 461 bp 1.0 78.5
clpP-psbB_p5 AGACGAAAAGGAACCTTCGG GCACAGCAATTAAACGACCC 50.0 50.0 57.8 58.0 462 bp 1.0 78.5