Hotspot

trnV-UAC-trnM-CAU

Genome · 53183-53370 · 188 bp

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Pi 0
MarkerSeek score 55.60
Primer available yes
Label trnV-UAC-trnM-CAU

Feature Metadata

label_name
trnV-UAC-trnM-CAU
feature_type
igs
region
Genome
length_bp
188
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-UAC-trnM-CAU_p1 TACCGCTGAGCTATAACCCT GCGATACTCCAACCACTGAG 50.0 55.0 57.6 58.1 232-238 bp 1.0 78.5
trnV-UAC-trnM-CAU_p2 ACAAGGAAACCGTGAATCGA GCGATACTCCAACCACTGAG 45.0 55.0 57.5 58.1 305-311 bp 1.0 76.7
trnV-UAC-trnM-CAU_p3 CGTGTAAACGAGGTGCTCTA GCGATACTCCAACCACTGAG 50.0 55.0 57.4 58.1 250-256 bp 1.0 76.3
trnV-UAC-trnM-CAU_p4 CACAAGGAAACCGTGAATCG GCGATACTCCAACCACTGAG 50.0 55.0 57.4 58.1 306-312 bp 1.0 76.2
trnV-UAC-trnM-CAU_p5 TCTCTGACAATCATTGGCGC GCGATACTCCAACCACTGAG 50.0 55.0 58.6 58.1 271-277 bp 1.0 76.2