Hotspot

psaM-trnS-GCU

Genome · 23011-23223 · 213 bp

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Pi 0
MarkerSeek score 54.30
Primer available yes
Label psaM-trnS-GCU

Feature Metadata

label_name
psaM-trnS-GCU
feature_type
igs
region
Genome
length_bp
213
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaM-trnS-GCU_p1 ACTCCAATCTCGGATAGCCA TAGTTGCCAGGAGAGAGAGG 50.0 55.0 57.9 57.8 401-405 bp 1.0 78.9
psaM-trnS-GCU_p2 CGTTTTCGCAACAGGTGTTT TAGTTGCCAGGAGAGAGAGG 45.0 55.0 58.1 57.8 360-364 bp 1.0 78.9
psaM-trnS-GCU_p3 ACTCCAATCTCGGATAGCCA GTGCTTTAGTTGCCAGGAGA 50.0 50.0 57.9 57.8 407-411 bp 1.0 78.6
psaM-trnS-GCU_p4 CGTTTTCGCAACAGGTGTTT GTGCTTTAGTTGCCAGGAGA 45.0 50.0 58.1 57.8 366-370 bp 1.0 78.6
psaM-trnS-GCU_p5 TTTCGCAACAGGTGTTTTCG TAGTTGCCAGGAGAGAGAGG 45.0 55.0 57.8 57.8 357-361 bp 1.0 78.2