Hotspot

ycf1

SSC · 116145-121514 · 5370 bp

Back to Pelatantheria

Pi 0.0204

MarkerSeek score 51.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5370
variable_sites: 109
pi: 0.0204
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGATTGCGACGAAAATCCGA	AGGTTTCTCGATGGTCATACA	45.0	42.9	58.0	56.5	235-253 bp	1.0	67.6
ycf1_p2	AGATTGCGACGAAAATCCGA	AAGGTTTCTCGATGGTCATACA	45.0	40.9	58.0	57.2	236-254 bp	1.0	65.3
ycf1_p3	AGATTGCGACGAAAATCCGA	AGGTTTCTCGATGGTCATACAA	45.0	40.9	58.0	57.2	235-253 bp	1.0	65.3
ycf1_p4	AGATTGCGACGAAAATCCGA	AAAAGGTTTCTCGATGGTCA	45.0	40.0	58.0	54.9	238-256 bp	1.0	63.0
ycf1_p5	AGATTGCGACGAAAATCCGA	GGTTTCTCGATGGTCATACA	45.0	45.0	58.0	54.9	234-252 bp	1.0	63.0