Pi
0.0951
MarkerSeek score
91.80
Primer available
yes
Label
ndhB
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ndhB_p1 | CATAACTCCAGCCCATTCCC | ATGGCAGAGGCAAATAGAGC | 55.0 | 50.0 | 58.0 | 58.0 | 1250-1409 bp | 1.0 | 88.2 |
| ndhB_p2 | CATAACTCCAGCCCATTCCC | GGCAGAGGCAAATAGAGCTT | 55.0 | 50.0 | 58.0 | 58.0 | 1248-1407 bp | 1.0 | 88.0 |
| ndhB_p3 | GCCCGAGTCAGACATCAAAT | ATGGCAGAGGCAAATAGAGC | 50.0 | 50.0 | 58.0 | 58.0 | 1600-1768 bp | 1.0 | 87.2 |
| ndhB_p4 | TGACAAAGGTGGAGTGTTGG | ATGGCAGAGGCAAATAGAGC | 50.0 | 50.0 | 57.9 | 58.0 | 1651-1819 bp | 1.0 | 86.9 |
| ndhB_p5 | CCGAGTGGGGATAGCTTTTC | ATGGCAGAGGCAAATAGAGC | 55.0 | 50.0 | 58.0 | 58.0 | 1239 bp | 0.5 | 59.5 |
| ndhB_p1 | CCTATTCATGGGGATTCCGT | CATAACTCCAGCCCATTCCC | 50.0 | 55.0 | 56.7 | 58.0 | 956-1132 bp | 1.0 | 79.6 |
| ndhB_p2 | TCCTATTCATGGGGATTCCGT | CATAACTCCAGCCCATTCCC | 47.6 | 55.0 | 58.3 | 58.0 | 957-1133 bp | 1.0 | 79.2 |
| ndhB_p3 | CCTATTCATGGGGATTCCGT | CTCCAGCCCATTCCCATTTT | 50.0 | 50.0 | 56.7 | 58.1 | 951-1127 bp | 1.0 | 78.7 |
| ndhB_p4 | TCCTATTCATGGGGATTCCGT | CTCCAGCCCATTCCCATTTT | 47.6 | 50.0 | 58.3 | 58.1 | 952-1128 bp | 1.0 | 78.3 |
| ndhB_p5 | TCCTATTCATGGGGATTCCG | CATAACTCCAGCCCATTCCC | 50.0 | 55.0 | 56.5 | 58.0 | 957-1133 bp | 1.0 | 77.3 |