Hotspot

rpl32-trnL-UAG

SSC · 110297-110808 · 512 bp

Back to Pelatantheria

Pi 0.0339
MarkerSeek score 69.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
512
variable_sites
17
pi
0.0339
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TCCACCGGACAGTCAAAAAG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.0 57.9 611-615 bp 1.0 82.4
rpl32-trnL-UAG_p2 TCCACCGGACAGTCAAAAAG AGCAGCGTGTCTACCAATTT 50.0 45.0 58.0 57.8 604-608 bp 1.0 81.9
rpl32-trnL-UAG_p3 TCCACCGGACAGTCAAAAAG CCTAAGAGCAGCGTGTCTAC 50.0 55.0 58.0 57.8 610-614 bp 1.0 81.8
rpl32-trnL-UAG_p4 TCCACCGGACAGTCAAAAAG CTAAGAGCAGCGTGTCTACC 50.0 55.0 58.0 57.8 609-613 bp 1.0 81.8
rpl32-trnL-UAG_p5 TCCACCGGACAGTCAAAAAG GAGCAGCGTGTCTACCAATT 50.0 50.0 58.0 58.3 605-609 bp 1.0 81.2