Hotspot

ycf1-rpl32

SSC · 109560-110122 · 563 bp

Back to Pelatantheria

Pi 0.0969

MarkerSeek score 80.40

Primer available yes

Label ycf1-rpl32

Feature Metadata

label_name: ycf1-rpl32
feature_type: igs
region: SSC
length_bp: 563
variable_sites: 47
pi: 0.0969
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rpl32_p1	TGCATGAAAATAATAAATTCGGT	GCTTTTACTGCCTCTAAAGATCC	26.1	43.5	53.4	57.3	678 bp	0.5	20.0
ycf1-rpl32_p2	TGCATGAAAATAATAAATTCGGT	AGAAAAAGCTTTTACTGCCTCT	26.1	36.4	53.4	56.2	685 bp	0.5	20.0
ycf1-rpl32_p3	TGCATGAAAATAATAAATTCGGT	AAGAAAAAGCTTTTACTGCCTCT	26.1	34.8	53.4	56.9	686 bp	0.5	20.0
ycf1-rpl32_p4	ATGCATGAAAATAATAAATTCGGT	GCTTTTACTGCCTCTAAAGATCC	25.0	43.5	54.0	57.3	679 bp	0.5	20.0
ycf1-rpl32_p5	ATGCATGAAAATAATAAATTCGGT	AGAAAAAGCTTTTACTGCCTCT	25.0	36.4	54.0	56.2	686 bp	0.5	20.0