Hotspot

cemA-petA

LSC · 61630-61875 · 246 bp

Back to Pelatantheria

Pi 0.0213
MarkerSeek score 68.50
Primer available yes
Label cemA-petA

Feature Metadata

label_name
cemA-petA
feature_type
igs
region
LSC
length_bp
246
variable_sites
5
pi
0.0213
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
cemA-petA_p1 CGTGTATCTCCTTCGCTTGT TGGATCGAGTCATCTGTTCT 50.0 45.0 57.7 55.7 335-346 bp 1.0 63.0
cemA-petA_p2 CGTGTATCTCCTTCGCTTGT TGGATCGAGTCATCTGTTCTT 50.0 42.9 57.7 56.5 335-346 bp 1.0 61.3
cemA-petA_p3 TCGTGTATCTCCTTCGCTTG TGGATCGAGTCATCTGTTCT 50.0 45.0 57.4 55.7 336-347 bp 1.0 60.7
cemA-petA_p4 CGTGTATCTCCTTCGCTTGT ATGGATCGAGTCATCTGTTCT 50.0 42.9 57.7 56.3 336-347 bp 1.0 59.5
cemA-petA_p5 CGTGTATCTCCTTCGCTTGT TGGATCGAGTCATCTGTTCTTT 50.0 40.9 57.7 57.2 335-346 bp 1.0 59.1