Hotspot

atpB-rbcL

LSC · 53596-54583 · 988 bp

Back to Pelatantheria

Pi 0.0149

MarkerSeek score 72.00

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 988
variable_sites: 14
pi: 0.0149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CGCCCTTGGTTTTGTTCTTC	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.9	58.1	1069-1088 bp	1.0	82.6
atpB-rbcL_p2	TGGGCCAATAATTTGAGCGA	GGTGACATAAGTCCCTCCCT	45.0	55.0	57.9	58.1	1091-1110 bp	1.0	82.5
atpB-rbcL_p3	TACGCCCTTGGTTTTGTTCT	GGTGACATAAGTCCCTCCCT	45.0	55.0	57.6	58.1	1071-1090 bp	1.0	80.9
atpB-rbcL_p4	CGCCCTTGGTTTTGTTCTTC	TGGTGACATAAGTCCCTCCC	50.0	55.0	57.9	58.4	1070-1089 bp	1.0	80.2
atpB-rbcL_p5	TGGGCCAATAATTTGAGCGA	TGGTGACATAAGTCCCTCCC	45.0	55.0	57.9	58.4	1092-1111 bp	1.0	80.1