Hotspot

psbM-trnD-GUC

LSC · 29492-30647 · 1156 bp

Back to Pelatantheria

Pi 0.0213

MarkerSeek score 73.30

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1156
variable_sites: 24
pi: 0.0213
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	CAGCGCAGTAGCAATAAACG	CCATGCATATGGCTGGGATT	50.0	50.0	57.9	58.1	1283-1303 bp	1.0	82.1
psbM-trnD-GUC_p2	CAGCGCAGTAGCAATAAACG	AGTTCAATCGGTCAGAGCAC	50.0	50.0	57.9	57.9	1261-1281 bp	1.0	82.0
psbM-trnD-GUC_p3	ATGAACAGCGCAGTAGCAAT	CCATGCATATGGCTGGGATT	45.0	50.0	58.3	58.1	1288-1308 bp	1.0	80.6
psbM-trnD-GUC_p4	TTGGCTGACTGTTTTGACGT	CCATGCATATGGCTGGGATT	45.0	50.0	58.3	58.1	1340-1360 bp	1.0	80.5
psbM-trnD-GUC_p5	TTGGCTGACTGTTTTGACGT	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.3	57.9	1318-1338 bp	1.0	80.4