Hotspot

trnR-UCU-atpA

LSC · 9753-9899 · 147 bp

Back to Pelatantheria

Pi 0.0479

MarkerSeek score 68.10

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 147
variable_sites: 7
pi: 0.0479
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	CCGAAGAAGCTGAAACCCTT	47.6	50.0	57.3	58.1	236-246 bp	1.0	71.3
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	TCACCGAAGAAGCTGAAACC	47.6	50.0	57.3	58.1	239-249 bp	1.0	71.1
trnR-UCU-atpA_p3	GTTCAAATCCTATTGGACGCA	CCGAAGAAGCTGAAACCCTT	42.9	50.0	56.6	58.1	235-245 bp	1.0	65.7
trnR-UCU-atpA_p4	GTTCAAATCCTATTGGACGCA	TCACCGAAGAAGCTGAAACC	42.9	50.0	56.6	58.1	238-248 bp	1.0	65.5
trnR-UCU-atpA_p5	GGTTCAAATCCTATTGGACGC	CACCGAAGAAGCTGAAACCC	47.6	55.0	57.3	59.1	238-248 bp	1.0	63.1