Hotspot

matK-trnK-UUU

LSC · 3313-4440 · 1128 bp

Back to Pelatantheria

Pi 0.0261

MarkerSeek score 67.00

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 1128
variable_sites: 24
pi: 0.0261
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TCCTGAAGGAGTAGCGGATA	TCCGGGTTGCTAACTCAATG	50.0	50.0	57.0	57.9	1078-1275 bp	1.0	76.4
matK-trnK-UUU_p2	TCCTGAAGGAGTAGCGGATA	CGGGTTGCTAACTCAATGGT	50.0	50.0	57.0	58.2	1076-1273 bp	1.0	75.8
matK-trnK-UUU_p3	TCCTGAAGGAGTAGCGGATA	GATCCGGGTTGCTAACTCAA	50.0	50.0	57.0	57.6	1080-1277 bp	1.0	74.1
matK-trnK-UUU_p4	TCCTGAAGGAGTAGCGGATA	TTGATCCGGGTTGCTAACTC	50.0	50.0	57.0	57.6	1082-1279 bp	1.0	74.1
matK-trnK-UUU_p5	TCCTGAAGGAGTAGCGGATAT	TCCGGGTTGCTAACTCAATG	47.6	50.0	57.5	57.9	1078-1275 bp	1.0	72.5