Hotspot

psbA-trnK-UUU

LSC · 1159-1410 · 252 bp

Back to Pelatantheria

Pi 0.0324

MarkerSeek score 70.30

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 252
variable_sites: 8
pi: 0.0324
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	TAGGCTTGTACTTTCGCGTC	TCGTGTGCAATGAAAACTGC	50.0	45.0	58.0	57.9	403-408 bp	1.0	81.5
psbA-trnK-UUU_p2	TAGGCTTGTACTTTCGCGTC	AAACTGCAAGCACGATTTGG	50.0	45.0	58.0	57.9	390-395 bp	1.0	81.4
psbA-trnK-UUU_p3	TAGGCTTGTACTTTCGCGTC	CGTGTGCAATGAAAACTGCA	50.0	45.0	58.0	58.2	402-407 bp	1.0	81.2
psbA-trnK-UUU_p4	TAGGCTTGTACTTTCGCGTC	ACGATTTGGGGAGGGATCTT	50.0	50.0	58.0	58.4	379-384 bp	1.0	79.4
psbA-trnK-UUU_p5	AACACCAAACCATCCGATGT	TCGTGTGCAATGAAAACTGC	45.0	45.0	57.7	57.9	469-474 bp	1.0	79.0