Pi
0.198
MarkerSeek score
84.00
Primer available
yes
Label
rpoA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| rpoA_p1 | TCTTGGAAGCTTTCGTCGAG | TTAGAAGTGGCGTCCGATTG | 50.0 | 50.0 | 57.9 | 58.0 | 1884-2072 bp | 0.615385 | 75.1 |
| rpoA_p2 | CCGATTCTTCCGTTCCCATT | TTAGAAGTGGCGTCCGATTG | 50.0 | 50.0 | 58.0 | 58.0 | 2229-2313 bp | 0.384615 | 64.8 |
| rpoA_p3 | CCGATTCTTCCGTTCCCATT | AAGGGATGCAGCATTACGAG | 50.0 | 50.0 | 58.0 | 58.0 | 2255-2339 bp | 0.384615 | 64.5 |
| rpoA_p4 | CCGATTCTTCCGTTCCCATT | GAAGGGATGCAGCATTACGA | 50.0 | 50.0 | 58.0 | 58.0 | 2256-2340 bp | 0.384615 | 64.4 |
| rpoA_p5 | ACAAGCAAAGCCCCTTCTAC | TTAGAAGTGGCGTCCGATTG | 50.0 | 50.0 | 58.1 | 58.0 | 1908-2096 bp | 0.307692 | 58.5 |
| rpoA_p1 | TTAGAAGTGGCGTCCGATTG | TCTTGGAAGCTTTCGTCGAG | 50.0 | 50.0 | 58.0 | 57.9 | 1884-2072 bp | 0.615385 | 75.1 |
| rpoA_p2 | TTAGAAGTGGCGTCCGATTG | CCGATTCTTCCGTTCCCATT | 50.0 | 50.0 | 58.0 | 58.0 | 2229-2313 bp | 0.384615 | 64.9 |
| rpoA_p3 | AAGGGATGCAGCATTACGAG | CCGATTCTTCCGTTCCCATT | 50.0 | 50.0 | 58.0 | 58.0 | 2255-2339 bp | 0.384615 | 64.6 |
| rpoA_p4 | GAAGGGATGCAGCATTACGA | CCGATTCTTCCGTTCCCATT | 50.0 | 50.0 | 58.0 | 58.0 | 2256-2340 bp | 0.384615 | 64.5 |
| rpoA_p5 | TTAGAAGTGGCGTCCGATTG | ACAAGCAAAGCCCCTTCTAC | 50.0 | 50.0 | 58.0 | 58.1 | 1908-2096 bp | 0.307692 | 58.5 |