Hotspot

ndhF

IRa · 147174-149408 · 2235 bp

Back to Pelargonium

Pi 0.164

MarkerSeek score 82.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: IRa
length_bp: 2235
variable_sites: 949
pi: 0.164
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGAAGAACCCCAGCTTTACC	GAGCTACTGAGGAACAACGG	50.0	55.0	57.4	58.0	2925-2928 bp	0.615385	60.7
ndhF_p2	TGAAGAACCCCAGCTTTACC	CCCGGAACTTCTTCGTAGTG	50.0	55.0	57.4	58.0	2830-2833 bp	0.615385	60.7
ndhF_p3	TGAAGAACCCCAGCTTTACC	CCGGAACTTCTTCGTAGTGG	50.0	55.0	57.4	58.0	2829-2832 bp	0.615385	60.7
ndhF_p4	TGAAGAACCCCAGCTTTACC	ACTCCCGGAACTTCTTCGTA	50.0	50.0	57.4	58.1	2833-2836 bp	0.615385	60.1
ndhF_p5	TGAAGAACCCCAGCTTTACC	TCCCGGAACTTCTTCGTAGT	50.0	50.0	57.4	58.1	2831-2834 bp	0.615385	60.1