Hotspot

psbE-petL

LSC · 77334-78203 · 870 bp

Back to Pelargonium

Pi 0.0506

MarkerSeek score 88.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 870
variable_sites: 138
pi: 0.0506
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 88.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AAGCGACGAGTAGAAAACCG	50.0	50.0	57.8	58.3	926-1039 bp	1.0	88.3
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	GCGACGAGTAGAAAACCGAA	50.0	50.0	57.8	58.3	924-1037 bp	1.0	88.3
psbE-petL_p3	GTTCTCCTGTGCTTCCAGAC	AAGCGACGAGTAGAAAACCG	55.0	50.0	58.2	58.3	921-1034 bp	1.0	88.0
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	GCGACGAGTAGAAAACCGAA	55.0	50.0	58.2	58.3	919-1032 bp	1.0	87.9
psbE-petL_p5	TCTCCTGTGCTTCCAGACAT	AAGCGACGAGTAGAAAACCG	50.0	50.0	58.4	58.3	919-1032 bp	1.0	86.8