Hotspot

trnD-GUC-trnG-GCC

LSC · 42205-42876 · 672 bp

Back to Pelargonium

Pi 0.364

MarkerSeek score 74.20

Primer available yes

Label trnD-GUC-trnG-GCC

Feature Metadata

label_name: trnD-GUC-trnG-GCC
feature_type: igs
region: LSC
length_bp: 672
variable_sites: 466
pi: 0.364
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.64
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnG-GCC_p1	ATGGATCTTGTGGCCGATTC	ACTTCACCTTCAACAAGCGT	50.0	45.0	58.0	58.0	1729-2440 bp	0.153846	56.0
trnD-GUC-trnG-GCC_p2	AACCGATTCTGCGAGACTTC	ACTTCACCTTCAACAAGCGT	50.0	45.0	58.0	58.0	1331 bp	0.076923	42.8
trnD-GUC-trnG-GCC_p3	AACCGATTCTGCGAGACTTC	TTCTCCTGGGCTTGTAGGAA	50.0	50.0	58.0	58.0	1179 bp	0.076923	42.7
trnD-GUC-trnG-GCC_p4	AACCGATTCTGCGAGACTTC	TTTCTCCTGGGCTTGTAGGA	50.0	50.0	58.0	58.0	1180 bp	0.076923	42.7
trnD-GUC-trnG-GCC_p5	AACCGATTCTGCGAGACTTC	TGAGGTTGCATTAATCGGGG	50.0	50.0	58.0	57.9	1389 bp	0.076923	42.6