Hotspot

psbM-trnD-GUC

LSC · 35660-36765 · 1106 bp

Back to Pelargonium

Pi 0.0582

MarkerSeek score 83.70

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1106
variable_sites: 186
pi: 0.0582
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TGCGAGAATATTGACTTCCA	TTCAATTGGTCAGAGCACCG	40.0	50.0	54.5	58.5	550-1271 bp	0.846154	53.9
psbM-trnD-GUC_p2	ATGCGAGAATATTGACTTCCA	TTCAATTGGTCAGAGCACCG	38.1	50.0	55.1	58.5	551-1272 bp	0.846154	50.8
psbM-trnD-GUC_p3	TGCGAGAATATTGACTTCCAT	TTCAATTGGTCAGAGCACCG	38.1	50.0	55.1	58.5	550-1271 bp	0.846154	50.8
psbM-trnD-GUC_p4	TGCGAGAATATTGACTTCCA	GTTCAATTGGTCAGAGCACC	40.0	50.0	54.5	57.0	551-1272 bp	0.846154	49.7
psbM-trnD-GUC_p5	AATGCGAGAATATTGACTTCCA	TTCAATTGGTCAGAGCACCG	36.4	50.0	55.9	58.5	552-1273 bp	0.846154	49.0