Hotspot

rpoB-trnC-GCA

LSC · 33790-34894 · 1105 bp

Back to Pelargonium

Pi 0.0457

MarkerSeek score 85.10

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1105
variable_sites: 173
pi: 0.0457
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 85.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCTTCATTTCTATCGCCAAGC	AGGATTTGCAGTCCCCCG	47.6	61.1	57.4	59.3	935-1190 bp	1.0	52.5
rpoB-trnC-GCA_p2	GGGATTGTAGCCATTCCTTCA	AGGATTTGCAGTCCCCCG	47.6	61.1	57.7	59.3	950-1205 bp	0.923077	52.0
rpoB-trnC-GCA_p3	AGGGATTGTAGCCATTCCTTC	AGGATTTGCAGTCCCCCG	47.6	61.1	57.4	59.3	951-1206 bp	0.923077	49.7
rpoB-trnC-GCA_p4	GGGATTGTAGCCATTCCTTCA	CTTACCGCTCGGCCATGT	47.6	61.1	57.7	59.8	931-1186 bp	0.923077	48.9
rpoB-trnC-GCA_p5	GGGATTGTAGCCATTCCTTCA	TTACCGCTCGGCCATGTC	47.6	61.1	57.7	59.8	930-1185 bp	0.923077	48.9