Hotspot

atpH-atpI

LSC · 20661-21598 · 938 bp

Back to Pelargonium

Pi 0.122

MarkerSeek score 85.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 938
variable_sites: 393
pi: 0.122
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 85.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACAGAAGCGGCAGAAATCA	ATGGAGGGTCATCATTGA	45.0	44.4	58.1	52.2	985-1242 bp	1.0	52.0
atpH-atpI_p2	GCAGAAATAACAGAAGCGGC	ATGGAGGGTCATCATTGA	50.0	44.4	57.8	52.2	993-1250 bp	1.0	52.0
atpH-atpI_p3	GGCCAACCCAGCAGAAATAA	ATGGAGGGTCATCATTGA	50.0	44.4	58.4	52.2	1003-1260 bp	1.0	52.0
atpH-atpI_p4	AGAAGCGGCAGAAATCAGTG	ATGGAGGGTCATCATTGA	50.0	44.4	58.6	52.2	982-1239 bp	1.0	52.0
atpH-atpI_p5	CAGAAGCGGCAGAAATCAGT	ATGGAGGGTCATCATTGA	50.0	44.4	58.6	52.2	983-1240 bp	1.0	52.0