Hotspot

trnS-GCU-trnG-UCC

LSC · 14874-15768 · 895 bp

Back to Pelargonium

Pi 0.053
MarkerSeek score 85.80
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
895
variable_sites
149
pi
0.053
unique_haplotype_count
13
primer_available
yes
alignment_reliability
0.99
markerseek_score
85.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA CACTTTTACCACTAAACTATACCCG 50.0 40.0 58.0 57.5 914-994 bp 1.0 52.0
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC CACTTTTACCACTAAACTATACCCG 50.0 40.0 58.0 57.5 913-993 bp 1.0 52.0
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA ACTTTTACCACTAAACTATACCCG 50.0 37.5 58.0 56.2 913-993 bp 1.0 52.0
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC ACTTTTACCACTAAACTATACCCG 50.0 37.5 58.0 56.2 912-992 bp 1.0 52.0
trnS-GCU-trnG-UCC_p5 AGCAATCCGACGCTTTAGTC CACTTTTACCACTAAACTATACCCG 50.0 40.0 58.4 57.5 916-996 bp 1.0 52.0