Hotspot

trnK-UUU-rps16

LSC · 12287-12894 · 608 bp

Back to Pelargonium

Pi 0.0577

MarkerSeek score 84.80

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 608
variable_sites: 112
pi: 0.0577
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CGGTCTTACAAACCCTACCG	GGATCTTCGAAAGGTGGGTC	55.0	55.0	58.0	58.0	892-905 bp	0.846154	85.2
trnK-UUU-rps16_p2	CCAACCCCTAATCGAATGGG	GGATCTTCGAAAGGTGGGTC	55.0	55.0	58.0	58.0	1240-1255 bp	0.846154	83.2
trnK-UUU-rps16_p3	GGTGGGGAATTTCTTGGGTT	GGATCTTCGAAAGGTGGGTC	50.0	55.0	58.0	58.0	1508-1520 bp	0.615385	70.2
trnK-UUU-rps16_p4	CGACGGTATGGACGAATCTC	GGATCTTCGAAAGGTGGGTC	55.0	55.0	58.0	58.0	875-887 bp	0.538462	68.8
trnK-UUU-rps16_p5	CTTACAAACCCTACCGACGG	GGATCTTCGAAAGGTGGGTC	55.0	55.0	58.0	58.0	889-900 bp	0.307692	56.0