Hotspot

rpoA

LSC · 20710-21807 · 1098 bp

Back to Pedinomonas

Pi 0.336

MarkerSeek score 80.50

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1098
variable_sites: 363
pi: 0.336
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	CCGATAAAGGTGCTTGAGCT	AAAGTACCCCATTTGCAGCA	50.0	45.0	58.0	58.0	1994 bp	0.5	59.7
rpoA_p2	CCGATAAAGGTGCTTGAGCT	AAGTACCCCATTTGCAGCAA	50.0	45.0	58.0	58.0	1993 bp	0.5	59.7
rpoA_p3	CCGATAAAGGTGCTTGAGCT	AGTACCCCATTTGCAGCAAA	50.0	45.0	58.0	58.0	1992 bp	0.5	59.7
rpoA_p4	TTCTTTTTCACGCGCATCAC	AAGTACCCCATTTGCAGCAA	45.0	45.0	58.0	58.0	2169 bp	0.5	59.7
rpoA_p5	TTCTTTTTCACGCGCATCAC	AGTACCCCATTTGCAGCAAA	45.0	45.0	58.0	58.0	2168 bp	0.5	59.7